reverse transcription super script ™ ii rt kit Search Results


rt pcr kit  (PCR Biosystems Ltd)
94
PCR Biosystems Ltd rt pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Rt Pcr Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+super+script+%E2%84%A2+ii+rt+kit/1-Step+Go+RT-PCR+Kit/pmc12818604-276-12-14
Average 94 stars, based on 1 article reviews
rt pcr kit - by Bioz Stars, 2026-09
94/100 stars
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first-strand cdna synthesis kit for rt-pcr improm-ii reverse transcription system  (Promega)
90
Promega first-strand cdna synthesis kit for rt-pcr improm-ii reverse transcription system
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
First Strand Cdna Synthesis Kit For Rt Pcr Improm Ii Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+super+script+%E2%84%A2+ii+rt+kit/first+strand+cdna+synthesis+kit+for+rt+pcr+improm+ii+reverse+transcription+system/pm19281268-127-15-21
Average 90 stars, based on 1 article reviews
first-strand cdna synthesis kit for rt-pcr improm-ii reverse transcription system - by Bioz Stars, 2026-09
90/100 stars
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primescript™ rt reagent kit impromm-ii reverse transcription system  (Promega)
90
Promega primescript™ rt reagent kit impromm-ii reverse transcription system
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Primescript™ Rt Reagent Kit Impromm Ii Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+super+script+%E2%84%A2+ii+rt+kit/primescript++rt+reagent+kit+impromm+ii+reverse+transcription+system/us10704099-154-1-5
Average 90 stars, based on 1 article reviews
primescript™ rt reagent kit impromm-ii reverse transcription system - by Bioz Stars, 2026-09
90/100 stars
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hiscript ii q rt supermix for qpcr  (Vazyme Biotech Co)
96
Vazyme Biotech Co hiscript ii q rt supermix for qpcr
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Hiscript Ii Q Rt Supermix For Qpcr, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+super+script+%E2%84%A2+ii+rt+kit/HiScript+II+Q+RT+SuperMix+for+qPCR/custom%40r223%409774183
Average 96 stars, based on 1 article reviews
hiscript ii q rt supermix for qpcr - by Bioz Stars, 2026-09
96/100 stars
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reverse transcription kit hiscript ii q rt  (Novozymes limited)
90
Novozymes limited reverse transcription kit hiscript ii q rt
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Reverse Transcription Kit Hiscript Ii Q Rt, supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+super+script+%E2%84%A2+ii+rt+kit/reverse+transcription+kit+hiscript+ii+q+rt/10__1016_slash_j__fbio__2025__107099-106-17-35
Average 90 stars, based on 1 article reviews
reverse transcription kit hiscript ii q rt - by Bioz Stars, 2026-09
90/100 stars
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omni script rt goscripttm reverse transcription kit  (Promega)
90
Promega omni script rt goscripttm reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Omni Script Rt Goscripttm Reverse Transcription Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+super+script+%E2%84%A2+ii+rt+kit/omni+script+rt+goscripttm+reverse+transcription+kit/pm25403906-42-9-16
Average 90 stars, based on 1 article reviews
omni script rt goscripttm reverse transcription kit - by Bioz Stars, 2026-09
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Image Search Results


(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Journal: PLOS Pathogens

Article Title: Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi

doi: 10.1371/journal.ppat.1013849

Figure Lengend Snippet: (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Article Snippet: All reactions were performed on the same plate, using PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems) following the recommended procedures.

Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Isolation, Tandem Mass Spectroscopy